在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:The role of galectin-1 in in vitro and in vivo photodynamic therapy with a galactodendritic porphyrin.
时间:2019-12-03 20:40:10
DOI:10.1016/j.ejca.2016.08.018
PMID:27718388
作者:Pereira, Patrícia M.R.; Silva, Sandrina; Ramalho, José S.
关键词:Galectin-1;Bladder cancer;Photodynamic therapy;Dendritic units of galactose;Porphyrin
出版源: 《European Journal of Cancer》 ,68 :60-69
摘要:•PorGal8, a porphyrin with dendritic units of galactose, has high affinity to galectin-1 protein.•PorGal8accumulation in cancer cells depends on the expression of galectin-1.•PorGal8induces phototoxicity in bladder cancer cells overexpressing galectin-1 protein.•Photodynamic therapy with PorGal8induces cytoskeleton disorganisation.•PorGal8exhibits anti-tumour activityin vivo.
大小:1872 kb
页数:11 PAGES
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

目录:
  • The role of galectin-1 in in vitro and in vivo photodynamic therapy with a galactodendritic porphyrin
    • 1. Introduction
    • 2. Materials and methods
      • 2.1. Synthesis of galactodendritic porphyrin (PorGal8)
      • 2.2. Galectin-1 interaction assays
      • 2.3. Cell treatments
      • 2.4. Cellular uptake of PorGal8, cell metabolic activity and membrane integrity, and apoptosis assay
      • 2.5. Mice and tumour cell implantation
      • 2.6. Western blot
      • 2.7. Fluorescence microscopy
      • 2.8. Statistical analysis
    • 3. Results
      • 3.1. PorGal8 interacts with galectin-1 and its accumulation in bladder cancer cells is dependent on the expression of this protein
      • 3.2. PorGal8 induces cytotoxicity after photodynamic activation
      • 3.3. PDT with PorGal8 induces ROS-mediated cytotoxicity and F-actin disorganisation
      • 3.4. PDT with PorGal8 induces tumour shrinkage
    • 4. Discussion
    • Conflict of interest statement
    • Acknowledgements
    • Appendix A. Supplementary data
    • References

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系