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标题:Label-free, zeptomole cancer biomarker detection by surface-enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles
时间:2019-12-03 09:40:54
DOI:10.1002/jbio.201400134
作者:Santos, Greggy M.; Zhao, Fusheng; Zeng, Jianbo
关键词:surface‐enhanced fluorescence;nanoporous gold disks;label‐free biosensor;zeptomole detection;molecular beacon
出版源: 《Journal of Biophotonics》 ,8 (10) :855-863
摘要:Abstract With patterned nanoporous gold disk (NPGD) plasmonic nanoparticles, a label-free biosensor that makes use of distance-dependent detection of surface-enhanced fluorescence (SEF) is constructed and tested for zeptomole detection of ERBB2 cancer gene DNA targets. We experimentally demonstrate a label-free biosensor for the ERBB2 cancer gene DNA target based on the distance-dependent detection of surface-enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non-porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.
大小:409 kb
页数:10 PAGES
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