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标题:Metatranscriptomic insights into microbial consortia driving methane metabolism in paddy soils
时间:2019-11-19 21:47:01
DOI:10.1080/00380768.2018.1457409
作者:Masuda, Yoko; Itoh, Hideomi; Shiratori, Yutaka
出版源: Soil Science and Plant Nutrition :1-10
摘要:Paddy soils have an environment in which waterlogging and drainage occur during the rice growing season. Fingerprinting analysis based on soil RNA indicated that active microbial populations changed in response to water management conditions, although the fundamental microbial community was stable as assessed by DNA-based fingerprinting analysis. Comparative clone library analysis based on... [Show full abstract]
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目录:
  • Abstract
  • 1. Introduction
  • 2. Materials and methods
    • 2.1. Study field and soil sampling
    • 2.2. Measurement of redox potential and Fe2+ content
    • 2.3. Preparation of soil RNA
    • 2.4. Shotgun RNA sequencing
    • 2.5. Informatics and statistics
    • 2.6. Nucleotide sequence accession number
  • 3. Results and discussion
    • 3.1. Seasonal and spatial profiles of soil redox properties and the potentials of methane metabolism
    • 3.2. Diversity of methanogens
    • 3.3. Diversity of methanotrophs
    • 3.4. Diversity of hydrogen-producing bacteria
    • 3.5. Diversity of acetogens
    • 3.6. Microbial consortia driving methane metabolism
  • Acknowledgments
  • Funding
  • References

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