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标题:Fermentation with Lactobacillus enhances the preventive effect of garlic extract on high fat diet-induced hepatic steatosis in mice
时间:2019-11-19 21:18:16
DOI:10.1016/j.jff.2016.12.043
作者:Lee, Hee-Seop; Lee, Sung-Jin; Yu, Heui-Jong
出版源: Journal of Functional Foods ,30 :125-133
摘要:This study aimed to investigate the effects of fermented garlic extract (FGE) by Lactobacillus plantarum BL2 in high fat diet (HFD)-induced hepatic steatosis. FGE administration increased insulin sensitivity and decreased serum lipid levels. Moreover, treatment with FGE effectively decreased hepatic lipid level, resulting in reduction of hepatic steatosis compared to that with non-fermented garlic extract (NFGE). These functions of FGE were associated with AMPK-mediated stimulation of hepatic lipolysis through upregulation of PPARα and suppression of hepatic lipogenesis, partly via downregulation of SREBP-1c, FAS, and SCD-1. Significant improvement in HFD-induced steatosis following administration of FGE was closely linked to its enhanced antioxidant activity of the enriched organosulfur compounds, cycloalliin and SAC, by fermentation, regarding hepatic redox status and oxidative damage. This study validated the advanced therapeutic value of FGE in the prevention of diet-induced non-alcoholic fatty liver disease.
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目录:
  • Fermentation with Lactobacillus enhances the preventive effect of garlic extract on high fat diet-induced hepatic steatosis in mice
    • 1 Introduction
    • 2 Materials and methods
      • 2.1 Chemicals and reagents
      • 2.2 Preparation of the cycloalliin-enriched fermented garlic extract
      • 2.3 OSC analysis and determination of antioxidant capacity
      • 2.4 Animal experiment
      • 2.5 Glucose tolerance test (GTT)
      • 2.6 Biochemical assays
      • 2.7 Hematoxylin and eosin, and immunohistochemical staining
      • 2.8 Immunoblot
      • 2.9 Detection of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and adenosine triphosphate (ATP)
      • 2.10 Statistical analysis
    • 3 Results
      • 3.1 Changes in OSCs in NFGE and FGE and antioxidant capacity
      • 3.2 Physiological role of FGE in mice on a HFD
      • 3.3 Effect of FGE on HFD-induced IR
      • 3.4 Effect of FGE on serum parameters in HFD-induced obese mice
      • 3.5 Effect of FGE on hepatic steatosis
      • 3.6 Improvement in hepatic lipid metabolism by FGE in HFD-induced obese mice
      • 3.7 Protection against HFD-induced hepatic steatosis by FGE through reduction of oxidative stress
    • 4 Discussion
    • Conflict of interest
    • Acknowledgements
    • References

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