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标题:Quantitative analysis of caveolin-rich lipid raft proteins from primary and metastatic colorectal cancer clones.
时间:2019-11-09 22:30:24
DOI:10.1016/j.jprot.2012.03.011
PMID:22484058
大小:1071 kb
页数:10 PAGES
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目录:
  • Quantitative analysis of caveolin-rich lipid raft proteins from primary and metastatic colorectal cancer clones
    • 1. Introduction
    • 2. Materials and methods
      • 2.1. Cell culture
      • 2.2. Enrichment of caveolin-rich lipid rafts
      • 2.3. Western blotting and densitometric protein quantification
      • 2.4. Enzymatic protein digestion
      • 2.5. Flow cytometry analysis
      • 2.6. LC/MS analysis
      • 2.7. Bioinformatics analysis
        • 2.7.1. Protein identification
        • 2.7.2. Differential protein expression
        • 2.7.3. Advanced bioinformatics analysis
    • 3. Results and discussion
    • 4. Conclusions
    • List of abbreviations
    • Competing interests
    • Authors' contributions
    • Acknowledgments
    • Appendix A. Supplementary data
    • References

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