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标题:Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma.
时间:2019-11-09 18:13:38
DOI:10.1016/j.cell.2019.10.003
PMID:31675496
大小:13200 kb
页数:39 PAGES
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目录:
  • Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma
    • Introduction
    • Results
      • High-Resolution Immune Landscape of HCC by Integrated Analysis of Full-Length and 3′ scRNA-Seq
      • Lymphocytes and Myeloid Cells in Ascites Appear to Have Distinct Origins
      • Two Distinct States of Tumor-Enriched Macrophages
      • LAMP3+ DCs: Mature DCs with Potential Migration Capacity in Tumors
      • Interaction Relationship of DCs and Lymphocytes
    • Discussion
    • Supplemental Information
    • Acknowledgments
    • Author Contributions
    • Declaration of Interests
    • References
    • STAR★Methods
      • Key Resources Table
      • Lead Contact and Materials Availability
      • Experimental Model and Subject Details
        • Human Specimens
      • Method Details
        • Sample collection and single cell processing
        • Flow cytometry of primary PBMC
        • In vitro stimulation of DC subsets
        • Single cell RNA-seq process for patients
        • Single cell RNA-seq process for in vitro DCs
        • In vitro stimulation of macrophages
        • Bulk RNA and DNA isolation and sequencing
        • Multi-color immunohistochemistry
        • Genetic knockout in THP-1 cells using CRISPR-Cas9
        • Multiplex cytokine assay using THP-1 cells
        • Dendritic cells migration assay
        • Single cell RNA-seq data processing
        • Processing bulk RNA-seq data
        • Dimension reduction and unsupervised clustering
        • Comparing DCs in different cancer types
        • Cell similarity analysis across tissues
        • TCR analysis
        • Lineage tracing based on mitochondrial mutation
        • RNA velocity-based cell fate tracing
        • Cell-cell interaction analysis
        • TCGA data analysis
      • Data and Code Availability

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