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标题:The Impact of Mitochondrial Fusion and Fission Modulation in Sporadic Parkinson’s Disease
时间:2019-10-23 17:27:50
DOI:10.1007/s12035-014-8893-4
大小:5986 kb
页数:15 PAGES
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目录:
  • The Impact of Mitochondrial Fusion and Fission Modulation in Sporadic Parkinson’s Disease
    • Abstract
    • Introduction
    • Materials and Methods
      • Chemicals
      • Human Subjects
      • Isolation of PBMCs
      • Preparation of Platelet Mitochondria
      • Creation of Cybrid Cell Lines
      • Cell Line Cultures and Treatments
      • Transfection Procedure
      • Preparation of Mitochondrial and Cytosolic Extracts
      • Immunocytochemistry and Confocal Microscopy Analysis
      • Western Blot Analysis
      • Fluorometric Reactive Oxygen Species Production
      • Analysis of Mitochondrial Membrane Potential (∆ψm) with MitoTracker Red
      • Analysis of Mitochondrial Membrane Potential (∆ψm) with Rhodamine 123 Probe
      • Data Analysis
    • Results
      • Mitochondrial Fragmentation and Altered Distribution in PD
      • Mitochondrial Fragmentation in mtDNA-Depleted Cells
      • Fusion and Fission Protein Expression in PD Patients’ PBMCs
      • MPP+-induced ROS Production Is Independent of OPA1 Mitochondrial Levels
      • Mitochondrial Fragmentation in PD Cybrids Occurs Through Drp1
    • Discussion
    • References

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