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标题:6-Hydroxydopamine (6-OHDA) induces Drp1-dependent mitochondrial fragmentation in SH-SY5Y cells
时间:2019-10-23 17:12:46
DOI:10.1016/j.freeradbiomed.2008.03.009
大小:1182 kb
页数:11 PAGES
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目录:
  • 6-Hydroxydopamine (6-OHDA) induces Drp1-dependent mitochondrial fragmentation in SH-SY5Y cells
    • Introduction
    • Materials and Methods
      • cDNAs and antibodies
      • Cell culture and drug treatment procedures
      • RNA interference and transfection experiments
      • Fluorescence microscopy
      • Mitochondrial morphology
      • Assessment of apoptotic cell death
      • Cytochrome c determination
      • Mitochondrial potential
      • Gel electrophoresis and immunoblotting
    • Results
      • 6-OHDA induces mitochondrial fragmentation in SH-SY5Y cells
      • Mitochondrial fragmentation precedes the collapse of mitochondrial membrane potential and cyto.....
      • Dynamin-like protein 1 is required for 6-OHDA-induced mitochondrial fragmentation
      • Bax and p53 are not involved in 6-OHDA-induced mitochondrial fission
    • Discussion
    • Acknowledgments
    • References

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