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标题:Endosperm-specific hypomethylation, and meiotic inheritance and variation of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) inter-strain hybrids.
时间:2019-10-10 17:15:27
DOI:10.1007/s00122-007-0555-8
PMID:17486309
大小:658 kb
页数:14 PAGES
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目录:
  • Endosperm-speciWc hypomethylation, and meiotic inheritance and variation of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) inter-strain hybrids
    • Abstract
      • Introduction
      • Materials and methods
        • Plant materials
        • DNA isolation
        • MSAP analysis
        • Recovery and sequencing of MSAP bands
        • Reverse transcriptase (RT)-PCR analysis
      • Results
        • DiVerence in cytosine methylation levels revealed by MSAP at the CCGG sites between leaf and endosperm, and between hybrids and inbred lines in sorghum
        • Inheritance and variation in locus-speciWc DNA methylation patterns revealed by MSAP in sorghum hybrids
        • The DNA methylation alteration in sorghum inter-strain hybrids appeared either non-random or stochastic and was not caused by parental heterozygosity
        • Sequences underlying DNA methylation alterations in sorghum inter-strain hybrids and sequences showing endosperm-speciWc hypomethylation
        • Endosperm-speciWc hypomethylation in some genic sequences is accompanied with increased transcript accumulation
      • Discussion
      • References

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