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标题:Kallistatin inhibits lymphangiogenesis and lymphatic metastasis of gastric cancer by downregulating VEGF-C expression and secretion.
时间:2019-10-10 17:07:33
DOI:10.1007/s10120-017-0787-5
PMID:29243194
大小:3582 kb
页数:16 PAGES
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目录:
  • Kallistatin inhibits lymphangiogenesis and lymphatic metastasis of gastric cancer by downregulating VEGF-C expression and secretion
    • Abstract
      • Background
      • Methods and results
      • Conclusions
    • Introduction
    • Materials and methods
      • Cell lines and cell culture
      • Tissue specimens and clinicopathological characteristics
      • Experimental animals
      • Popliteal lymph node metastasis model
      • Expression and purification of recombinant kallistatin
      • Enzyme-linked immunosorbent assay (ELISA)
      • Hematoxylin and eosin (H&E) staining and immunohistochemistry
      • Cytoplasmic and nuclear protein extraction
      • Western blotting
      • siRNA transfection
      • Quantitative (q)RT-PCR
      • Immunofluorescence
      • Co-immunoprecipitation (Co-IP) assay
      • Chromatin immunoprecipitation assay
      • Luciferase assay
      • Statistical analysis
    • Results
      • Kallistatin was reduced in the cancer tissue and plasma of gastric cancer patients
      • Kallistatin was related to the lymph node metastasis of gastric cancer
      • Kallistatin inhibited the growth and lymphatic metastasis of gastric cancer in vivo
      • Expression and secretion of kallistatin and VEGF-C were negatively correlative
      • Kallistatin downregulated the expression and secretion of VEGF-C in gastric cancer cells
      • The NF-ҡB pathway is involved in the downregulation of VEGF-C expression by kallistatin
      • LRP6 served as a receptor to induce the inhibitory effect of kallistatin
    • Discussion
    • Acknowledgements
    • References

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