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标题:Overexpression of AKIP1 promotes angiogenesis and lymphangiogenesis in human esophageal squamous cell carcinoma.
时间:2019-10-10 16:41:32
大小:3323 kb
页数:11 PAGES
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目录:
  • title_link
    • Introduction
    • Results
      • Overexpression of AKIP1 correlates with progression and poor prognosis in ESCC
      • AKIP1 induces ESCC angiogenesis and lymphangiogenesis in™vivo and in™vitro
    • Figure™1AKIP1 is overexpressed in ESCC cell lines and primary human ESCC. (a, b) Western blot analysis of AKIP1 expression in 2 normal esophageal epithelial cells (NEECs) and 12 cultured ESCC cell lines (a), and in 10 paired human ESCC tissues (T) and the
      • VEGF-C is essential for AKIP1-induced angiogenesissollymphangiogenesis
    • Figure™2AKIP1 promotes ESCC angiogenesis and lymphangiogenesis in™vivo. (a) Western blot analysis of AKIP1 expression in Kyse30 (left panel) and Kyse 510 (right panel) ESCC cells stably expressing AKIP1-complementary DNA or AKIP1-short hairpin RNA(s). agr
      • AKIP1 regulates VEGF-C promoter activity in ESCC cells
    • Figure™3VEGF-C is essential for AKIP1-induced angiogenesissollymphangiogenesis. (a) Real-time PCR analysis of VEGF-C mRNA expression in vector control cells and AKIP1-transduced ESCC cells, or RNAi-vector control cells and AKIP1-silenced ESCC cells. Expre
    • Figure™4VEGF-C contributes to AKIP1-induced angiogenesissollymphangiogenesis in™vivo. (a) Representative images of the tumor-bearing mice. (b, c) IHC staining demonstrating that silencing VEGF-C abrogated the stimulatory effect of AKIP1 on angiogenesis an
    • Figure™5AKIP1 regulates VEGF-C promoter activity in ESCC cells. (a) Transactivation of the VEGF-C promoter by AKIP1 (left panel) and repression of the VEGF-C promoter by AKIP1- small interfering RNA (right panel) in the indicated ESCC cells, as demonstrat
      • Clinical relevance of AKIP1 and VEGF-C expression in human ESCC
    • Figure™6Clinical relevance of AKIP1 and VEGF-C expression in human ESCC. (a) AKIP1 levels were positively associated with VEGF-C expression in 301 primary human ESCC specimens. Left panel: two representative cases are shown. Original magnification, times2
    • Discussion
    • Materials and methods
      • Cells
      • Patient information and tissue specimens
      • Vectors, retroviral infection and transfection
      • Immunohistochemistry
      • Xenografted tumor model, IHC, and hematoxylin and eosinstaining
      • Western blot assay
      • Migration assay
      • Tube formation assay
      • Enzyme-linked immunosorbent assay
      • Luciferase assay
      • IP assay
      • Chromatin IP assay
      • Statistical analysis
    • A5
    • A6
    • ACKNOWLEDGEMENTS
    • A7

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