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标题:In vitro antimetastatic activity of Agarwood (Aquilaria crassna) essential oils against pancreatic cancer cells
时间:2019-09-12 13:37:14
DOI:10.1016/j.ajme.2015.07.001
作者:Saad Sabbar Dahham;Yasser M. Tabana;Loiy E. Ahmed Hassan;Mohamed B. Khadeer Ahamed;Aman Shah Abdul Majid
出版源: 《Alexandria Journal of Medicine》 ,2016 ,52 (2) :141-150
摘要:In vitro antimetastatic activity of Agarwood ( Aquilaria crassna ) essential oils against pancreatic cancer cellsEssential oilsAgarwood...
大小:2676 kb
页数:11 PAGES
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目录:
  • In vitro antimetastatic activity of Agarwood (Aquilaria crassna) essential oils against pancreatic cancer cells
    • 1 Introduction
    • 2 Materials and methods
      • 2.1 Plants material and extraction of essential oils
      • 2.2 Characterization techniques
        • 2.2.1 FT-IR
        • 2.2.2 Gas chromatography–mass (GC–MS) spectral analysis of the extract
      • 2.3 Chemicals and reagents
      • 2.4 Cell lines and culture conditions
      • 2.5 Cell viability assay
      • 2.6 Cell migration assay
      • 2.7 Colony formation assay
      • 2.8 Determination of nuclear condensation and mitochondrial membrane potential
      • 2.9 Statistical analysis
    • 3 Results
      • 3.1 Characterization of Agarwood essential oils using FT-IR and GC–MS
      • 3.2 Effect of EOs on the cell viability
      • 3.3 Eos inhibited cell migration
      • 3.4 EOs inhibited MIA PaCa-2 colony formation
      • 3.5 EOs induced nuclear morphological changes of MIA PaCa-2 cells
      • 3.6 Eos induced loss of the mitochondrial membrane potential in MIA PaCa-2 cells
    • 4 Discussion
    • 5 Conclusion
    • Conflict of interest
    • Acknowledgments
    • References

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