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标题:Mediationofexogenoushydrogensulfideinrecoveryofischemicpost-conditioning-inducedcardioprotectionviadown-regulatingoxidativestressand...
时间:2019-09-11 17:38:08
作者:LIHong-zhu;WANGYue-hong;WEICan;BAIShu-zhi;ZHAOYa-jun
出版源: 《中国病理生理杂志》 ,2015 ,5 (10) :1-14
摘要:正AIM:To investigate the role of hydrogen sulfide(H2S)in the recovery of ischemic post-conditioning(PC)-induced cardioprotection in the aging hearts.METHOD...
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页数:15 PAGES
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目录:
  • Abstract
  • Introduction
  • Materials and methods
    • Materials
    • Isolated heart preparation
    • Experimental protocols
    • Determination of H2S production
    • Measurement of MDA level, LDH, CK and SOD activities
    • Reactive oxygen species (ROS) level analysis
    • Myocardial ultrastructure measurement
    • Observation of myocardial infarct size
    • Cardiac function evaluation
    • Apoptosis assay by TUNEL Staining
    • Real-Time PCR analysis
    • Detection of Cyt c release from mitochondrial
    • Western blotting analysis
    • Statistical analysis
  • Results
    • H2S production rate and CSE expressions in both young and aging hearts
    • Exogenous H2S attenuates myocardial tissue injury
    • Exogenous H2S improves cardiac function
    • Exogenous H2S decreases the rate of apoptotic cell
    • Exogenous H2S increases the level of Bcl-2 mRNA, decreases the level of caspase-3 and caspase-9 mRNA and Cyt c release
    • Exogenous H2S inhibits SOD activity and decreases MDA content and ROS level
    • Exogenous H2S up-regulates PI3K–Akt–GSK-3β pathway
  • Discussion
  • Additional file
  • Competing interests
  • Authors’ contributions
  • Acknowledgements
  • Author details
  • References

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