在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:Single-molecule detection of protein efflux from microorganisms using fluorescent single-walled carbon nanotube sensor arrays.
时间:2019-09-11 17:13:33
DOI:10.1038/nnano.2016.284
PMID:28114298
作者:Markita Patricia Landry;Hiroki Ando;Allen Y. Chen;Jicong Cao;Vishal Isaac Kottadiel
出版源: 《Nature Nanotechnology》 ,2017 ,12 (4) :368-377
摘要:Single-molecule detection of protein efflux from microorganisms using fluorescent single-walled carbon nanotube sensor arraysAuthor(s): Landry, MP; Ando, H; Chen, AY; Cao, J; Kottadiel, VI; Chio, L; Yang, D; Dong, J; Lu, TK...
大小:6859 kb
页数:11 PAGES
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

目录:
  • Single-molecule detection of protein efflux from microorganisms using fluorescent single-walled carbon nanotube sensor arrays
  • Aptamer-anchor design for selective protein recognition
  • Unlabelled protein detection from crude cell lysates
  • Real-time detection of protein secreted from E. coli
  • „Protein detection from single E. coli, HEK 293 and P. pastoris cells
  • Real-time detection of virally induced cell lysis
  • Conclusions
  • Figure 1 Characterization of the aptamer-anchor structure on nanotube.
  • Figure 2 Calibration of nanosensor response to recombinant protein.
  • Figure 3 Detection of protein from crude cell lysate and from E. coli engineered to secrete target protein.
  • Figure 4 Imaging the secretion of single proteins from individual microorganisms.
  • Figure 5 Real-time monitoring of bacteriophage T7RAP1 infection of E. coli cells and resulting cell lysis.
  • References
  • Acknowledgements
  • Author contributions
  • Additional information
  • Competing financial interests

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系