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标题:Mature miR-183, negatively regulated by transcription factor GATA3, promotes 3T3-L1 adipogenesis through inhibition of the canonical Wnt/β-catenin signaling pathway by targeting LRP6.
时间:2019-09-11 16:49:57
DOI:10.1016/j.cellsig.2014.02.003
PMID:24556500
作者:Chen C;Xiang H;Peng YL;Peng J;Jiang SW
出版源: 《Cellular Signalling》 ,2014 ,26 (6) :1155-1165
摘要:Mature miR-183, negatively regulated by transcription factor GATA3, promotes 3T3-L1 adipogenesis through inhibition of the canonical Wnt/β-catenin signaling ...
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目录:
  • Mature miR-183, negatively regulated by transcription factor GATA3, promotes 3T3-L1 adipogenesis through inhibition of the canonical Wnt/ β-catenin signaling pathway by targeting LRP6
    • 1. Introduction
    • 2. Materials and methods
      • 2.1. Cell culture, differentiation and miR-183 oligonucleotide transfection
      • 2.2. QPCR analysis
      • 2.3. Western blot analysis
      • 2.4. Oil Red O staining and triglyceride content assays
      • 2.5. Bioinformatics analysis of mouse miR-183 gene
      • 2.6. Luciferase vector construction and reporter assays
      • 2.7. Chromatin immunoprecipitation (ChIP)
      • 2.8. Statistical analysis
    • 3. Results
      • 3.1. miR-183 regulated 3T3-L1 preadipocyte differentiation and adipogenesis
      • 3.2. miR-183 inhibited the canonical Wnt/β-catenin signaling pathway by targeting LRP6
      • 3.3. LRP6 down-regulation affected 3T3-L1 preadipocyte differentiation and adipogenesis
      • 3.4. Bioinformatics analysis of mouse miR-183 gene structure
      • 3.5. Identification of the core promoter of mouse miR-183
      • 3.6. The binding of transcription factor GATA3 to the core promoter of mouse miR-183
      • 3.7. The up-regulation of LRP6 expression by transcription factor GATA3
    • 4. Discussion
    • Conflict of interest statement
    • Acknowledgments
    • References

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