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标题:Claudin expression in the rat endolymphatic duct and sac - first insights into regulation of the paracellular barrier by vasopressin.
时间:2019-07-12 21:02:41
DOI:10.1038/srep45482
PMID:28374851
作者:Runggaldier, Daniel;Pradas, Lidia Garcia;Neckel, Peter H.;Mack, Andreas F.;Hirt, Bernhard
出版源: 《Sci Rep》 ,2017 ,7 :45482
摘要:Claudin expression in the rat endolymphatic duct and sac - first insights into regulation of the paracellular barrier by vasopressinAbstract Hearing and balance functions of the inner ear rely on the homeostasis of the endolymphatic fluid. ...
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页数:18 PAGES
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目录:
  • Claudin expression in the rat endolymphatic duct and sac - first insights into regulation of the paracellular barrier by va ...
    • Results
      • Different ultrastructural morphology of TJs in the ED and ES epithelium.
      • Distinct mRNA expression of claudin genes in the ES and ED epithelium.
      • Differences in the expression of Claudin 4, 8 and 16 between the ED and ES epithelium.
      • Differential subcellular expression of Claudin 3 in the ED and ES epithelium.
      • Subcellular localization of Claudin 6, 7, and 10 in the ED and ES epithelium.
      • Synthetic arginine-vasopressin (AVP) analogon 1-Desamino-8-D-Arginin-Vasopressin (dDAVP) induces the translocation of Claud ...
    • Discussion
    • Material and Methods
      • Animals and tissue preparation.
      • Transmission electron microscopy (TEM).
      • RT-PCR and high throughput real-time PCR of ED and ES preparations.
      • qPCR data evaluation and statistical analysis.
      • Immunofluorescence microscopy.
      • Pharmacological AVP stimulation of the ED and ES.
    • Acknowledgements
    • Author Contributions
    • Figure 1.  Schematic illustration of the membranous labyrinth of the inner ear represents the endolymphatic duct (ED green) and sac (ES green) and its relationship to the cochlea (red) and the vestibule (utricle and semicircular canals blue).
    • Figure 2.  Transmission electron microscopy of the endolymphatic epithelia of rat p4 ED, PSP/ISP and DSP.
    • Figure 3.  Claudin mRNA expression in rat p4 ED and ES epithelium.
    • Figure 4.  Cellular expression of claudin proteins in the ED and ES epithelium of p4 rat.
    • Figure 5.  cLSM analysis of the subcellular immunolocalization of Claudin 4 and 6 in rat p4 epithelial cells of ED and ES.
    • Figure 6.  cLSM analysis of Claudin 3 labeling in cryo-sections of the rat p4 ED and ES epithelium.
    • Figure 7.  cLSM analysis of the subcellular immunolocalization of Claudin 6, 7 and 10 in the epithelial cells of p4 rat.
    • Figure 8.  Immunolabeling of dDAVP induces the translocation of Claudin 4 into the TJs of the ES epithelia cells.
    • Table 1.  Assesment of absolute Claudin mRNA expression in rat p4 ED, ES and ST.
    • Table 2.  TaqMan Gene Expression Assays (Thermo Fisher Scientific).
    • Table 3.  Antibodies.

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