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标题:Genotyping and development of single-nucleotide polymorphism (SNP) markers associated with blast resistance genes in rice using GoldenGate assay
时间:2019-07-12 14:26:40
DOI:10.1007/s11032-014-0129-9
作者:Shallu Thakur;Pankaj K. Singh;Rajeev Rathour;Mukund Variar;Sangam K. Prashanthi
出版源: 《Molecular Breeding》 ,2014 ,34 (3) :1449-1463
摘要:Genotyping and development of single-nucleotide polymorphism (SNP) markers associated with blast resistance genes in rice using GoldenGate assayIllumina GoldenGate assayCAPSBlast resistance geneMagnaporthe oryzaeRiceDevelopment and large-scale genotyping of single-nucleotide polymorphism (SNP) is required to use identified sequence variation in the alleles of different genes to ...
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目录:
  • Genotyping and development of single-nucleotide polymorphism (SNP) markers associated with blast resistance genes in rice using GoldenGate assay
    • Abstract
    • Introduction
    • Materials and methods
      • Plant materials
      • Identification of SNPs from the blast resistance genes
      • Selection of SNPs for genotyping
      • Designing of oligo pool assay (OPA)
      • SNPs genotyping in a set of rice lines
      • Analysis of genetic structure of the population
      • Validation of SNPs through CAPS analysis and development of CAPS markers
    • Results and discussion
      • Success rate of GoldenGate assay
      • SNP validation
      • Analysis of allele frequencies
      • Assessment of the molecular genetic diversity
      • Analysis of population genetic structure
      • Development of cleaved amplified polymorphic sequence (CAPS) marker
    • Acknowledgments
    • References

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