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标题:Development of a viability standard curve for microencapsulated probiotic bacteria using confocal microscopy and image analysis software.
时间:2019-07-12 13:42:30
DOI:10.1016/j.mimet.2015.04.002
PMID:25887694
作者:Sarah, Moore;Kasipathy, Kailasapathy;Michael, Phillips;Mark R, Jones
出版源: 《Journal of Microbiological Methods》 ,2015 ,114 :16-22
摘要:•Viability standard curve for microencapsulated bacteria using confocal microscopy, fluorescent dyes and image analysis.•Real-time viability of micro...
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目录:
  • Development of a viability standard curve for microencapsulated probiotic bacteria using confocal microscopy and image anal...
    • 1. Introduction
    • 2. Materials and methods
      • 2.1. Bacterial strains and media
      • 2.2. Microencapsulation materials
      • 2.3. Staining of bacteria with LIVE/DEAD® BacLight™ Bacterial Viability and Counting Kit (Invitrogen)
      • 2.4. Microencapsulation of bacteria
      • 2.5. Laser scanning confocal microscopy (LSCM) analysis of free & microencapsulated bacteria and empty capsules
      • 2.6. Analysis of microencapsulated bacteria viability using the Bitplane Imaris software
      • 2.7. Statistics
    • 3. Results
      • 3.1. Determining the distribution of probiotic bacteria cells in capsules using laser scanning confocal microscopy analysis
      • 3.2. Analysis and development of a viability standard curve for microencapsulated bacteria using confocal microscopy and th...
    • 4. Discussion
      • 4.1. Distribution of microencapsulated probiotic bacteria cells
      • 4.2. Viability standard curve of microencapsulated bacteria
    • 5. Conclusion
    • 6. Uncited references
    • Acknowledgements
    • References

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