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标题:The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer.
时间:2019-07-12 00:08:00
DOI:10.1186/s40425-019-0521-4
PMID:30760333
大小:1909 kb
页数:14 PAGES
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目录:
  • Abstract
    • Background
    • Methods
    • Results
    • Conclusions
  • Background
  • Methods
    • Cells and culture media
    • Real-time PCR in tumor cells
    • siRNA transfection
    • Cytokine quantification in tumor cells
    • CAF stimulation
    • In vivo experiments
    • Western blot analysis
    • Tumor cell supernatant precipitation
    • Immunofluorescence
    • Macrophage stimulation
    • Immunohistochemistry
    • Statistical analysis and bioinformatics
  • Results
    • TSLP secretion by CAFs induced by tumor cell supernatant mainly depends on IL-1α
    • Tumor cells release alarmins, among which ASC, for inflammasome activation and IL-1β secretion by macrophages
  • Discussion
  • Conclusions
  • Additional files
  • Abbreviations
  • Acknowledgements
  • Funding
  • Availability of data and materials
  • Authors’ contributions
  • Ethics approval and consent to participate
  • Consent for publication
  • Competing interests
  • Publisher’s Note
  • Author details
  • References

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