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标题:A pathway for biological methane production using bacterial iron-only nitrogenase.
时间:2019-07-11 22:17:26
DOI:10.1038/s41564-017-0091-5
PMID:29335552
作者:Zheng Y;Harris DF;Yu Z;Fu Y;Poudel S
出版源: 《Nature Microbiology》 ,2018 ,3 (3)
摘要:A pathway for biological methane production using bacterial iron-only nitrogenaseNitrogenase catalyzes the MgATP-dependent reduction of dinitrogen gas to ammonia. In addition to the physiological substrate, nitrogenase catalyzes reduction of a variety of other multiply bonded substrates, such as acetylene, nitrous oxide, and azide. Although carbon monoxide (CO) is not reduced by...
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目录:
  • A pathway for biological methane production using bacterial iron-only nitrogenase
    • Methods
      • Bacterial strains and growth conditions
      • Genetic manipulation of R. palustris
      • CH4 measurements from whole cells
      • Capillary gas chromatography mass spectrometry analysis for in vivo CO2 reduction to CH4 by R. palustris
      • Nitrogenase purification
      • CO2 reduction assays
      • N2 and H+ reduction assays
      • Identification and compilation of nitrogenases homologues
      • Co-culture of R. palustris CGA010 and Methylomonas sp. LW13
      • Liquid chromatography–tandem mass spectrometry analysis for CH4 uptake by Methylomonas sp. LW13
      • Life Sciences Reporting Summary
      • Data availability
    • Acknowledgements
    • Fig. 1 Cells expressing wild-type Fe-only nitrogenase produce CH4 a, CH4 production by non-growing cell suspensions of R.
    • Fig. 2 Fe-only nitrogenase purified from R.
    • Fig. 3 Effect of molybdenum on CH4 production by nitrogen-fixing bacteria.
    • Fig. 4 Co-culture of Methylomonas sp.

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