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标题:A proteomic insight into the MSP1 and flg22 induced signaling in Oryza sativa leaves.
时间:2019-06-13 09:03:25
DOI:10.1016/j.jprot.2018.04.015
PMID:29970347
作者:Meng Q;Gupta R;Min CW;Kim J;Kramer K
出版源: 《Journal of Proteomics》 ,2018 :S1874391918301751-
摘要:A proteomic insight into the MSP1 and flg22 induced signaling in Oryza sativa leavesMSP1 is a M. oryzae secreted protein, which triggers defense responses in rice. Previous reports have shown that MSP1 is required for the pathogenicity of rice blast fungus, however, the exact mechanism of its action and its downstream targets in rice are currently unknown. ...
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目录:
  • A proteomic insight into the MSP1 and flg22 induced signaling in Oryza sativa leaves
    • Introduction
    • Materials and methods
      • Plant material and growth conditions
      • Sample preparation
      • MAPKs assay
      • Oxidative burst measurement
      • Protein extraction and enrichment of low-abundance proteins
      • Sample preparation and LC-MS/MS for proteomic analysis
      • LC-MS/MS data analysis for proteomic comparisons
      • Bioinformatics analysis
      • RNA isolation and qPCR analysis
    • Results
      • MSP1 triggers immune responses in rice
      • Overview of differentially modulated proteins in response to MP1 and flg22 treatments
      • Functional annotation of differentially modulated proteins
      • Protein-protein interaction (PPI) network of differentially modulated proteins in response to MSP1 treatment
      • qPCR analysis revealed that many protein abundance changes are caused by regulation of the expression patterns of the identified proteins
    • Discussion
      • Reprogramming of proteome by MSP1 and flg22 treatments
      • Activation of signaling and defense response pathways by both MSP1 and flg22 treatments
      • Accumulation of proteins related to redox regulation in response to MSP1 and flg22 treatments
      • Changes in protein turnover and degradation upon MSP1 and flg22 treatments
    • Conclusion
    • Author contributions
    • Conflict of interest
    • Notes
    • Acknowledgments
    • References

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