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标题:Essential role of METTL3-mediated m6A modification in glioma stem-like cells maintenance and radioresistance.
时间:2019-06-12 18:47:39
DOI:10.1038/onc.2017.351
PMID:28991227
作者:A Arivazhagan;A Arora;A S Hegde;A Visvanathan;K Somasundaram
出版源: 《Oncogene》 ,2018 ,37 (4)
摘要:Visvanathan, A.; Patil, V.; Arora, A.; Hegde, A.S.; Arivazhagan, A.; Santosh, V.; Somasundaram, K. Essential role of METTL3-mediated m(6)A modification in glioma stem-like cells maintenance and radioresistance. Oncogene ...
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目录:
  • Essential role of METTL3-mediated m6A modification in glioma stem-like cells maintenance and radioresistance
    • Introduction
    • Results
      • Elevated METTL3 levels observed in GSCs is essential for GSC maintenance
    • Figure 1 METTL3 is upregulated in GSC over DGC and essential for stem cell maintenance.
    • Figure 2 m6A methylation by METTL3 regulates SOX2 mRNA stability.
    • Figure 3 METTL3 interacts with SOX2 mRNA in’vivo and in’vitro via 3′UTR.
      • SOX2 mediates the METTL3-dependent GSC maintenance
      • METTL3 interacts and methylates specific sites of SOX2-3′UTR
      • Recruitment of HuR to m6A-modified sites is essential for SOX2 mRNA stabilization
    • Figure 4 HuR is essential in METTL3-dependent SOX2 mRNA stabilization.
      • METTL3 imparts radioresistance in GSCs through SOX2-dependent enhanced DNA repair
    • Figure 5 Silencing METTL3 radiosensitizes GSCs by interruption of SOX2-dependent DNA repair.
    • Figure 6 METTL3 silencing diminish in’vivo tumor propagation.
      • METTL3 is upregulated in GBM and its silencing in GSCs suppresses tumor growth in’vivo
    • Discussion
    • Figure 7 METTL3 imparts stemness and radioresistance in GBM.
    • Materials and methods
      • Tumor samples and establishing neurosphere lines from primary GBM tissue
      • GSCs culture
      • Other experimental methods
    • The results published here are in part based upon data generated by The Cancer Genome Atlas (TCGA) pilot project established by the NCI and NHGRI. Information about TCGA and the investigators and institutions, which constitute the TCGA research network, c
    • The results published here are in part based upon data generated by The Cancer Genome Atlas (TCGA) pilot project established by the NCI and NHGRI. Information about TCGA and the investigators and institutions, which constitute the TCGA research network, c
    • ACKNOWLEDGEMENTS
    • REFERENCES

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