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标题:Gambogenic acid reverses P-glycoprotein mediated multidrug resistance in HepG2/Adr cells and its underlying mechanism.
时间:2019-05-17 03:02:52
DOI:10.1016/j.bbrc.2018.12.028
PMID:30538042
作者:Xu, Qianqian;Guo, Junsong;Chen, Weidong
出版源: Biochemical and Biophysical Research Communicat...
摘要:Gambogenic acid reverses P-glycoprotein mediated multidrug resistance in HepG2/Adr cells and its underlying mechanismdoi:10.1016/j.bbrc.2018.12.028Biochemical and Biophysical Research CommunicationsXu, QianqianGuo, JunsongChen, Weidong...
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目录:
  • Gambogenic acid reverses P-glycoprotein mediated multidrug resistance in HepG2/Adr cells and its underlying mechanism
    • 1. Introduction
    • 2. Materials and methods
      • 2.1. Reagents
      • 2.2. Cell culture
      • 2.3. Cell cytotoxicity assay
      • 2.4. Apoptosis analysis
      • 2.5. DOX and Rho-123 accumulation assay
      • 2.6. Intracellular DOX and Rho-123 accumulation assay
      • 2.7. P-gp ATPase assay
      • 2.8. RT-qPCR (reverse transcription-quantitative PCR) analysis
      • 2.9. Western blot analysis
      • 2.10. Statistical analysis
    • 3. Results
      • 3.1. GNA potentiated the cell cytotoxicity of the anticancer drugs in HepG2/Adr cells and promoted the apoptosis of HepG2/Adr cells
      • 3.2. GNA increased the DOX and Rho-123 accumulation in HepG2/Adr cells, while had no effect on the ATPase activity of P-gp
      • 3.3. GNA changed the levels of P-gp and P-gp-related proteins in the HepG2/Adr and HepG2 cell lines
    • 4. Discussion
    • Acknowledgements
    • Transparency document
    • Appendix A. Supplementary data
    • References

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