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标题:Fabrication of electrospun silk fibroin scaffolds coated with graphene oxide and reduced graphene for applications in biomedicine.
时间:2019-05-16 14:48:06
DOI:10.1016/j.bioelechem.2015.12.003
PMID:26717014
作者:Aznar-Cervantes S;Martínez JG;Bernabeu-Esclapez A;Lozano-Pérez AA;Meseguer-Olmo L
出版源: 《Bioelectrochemistry》 ,2016 ,108 :36-45
摘要:The conductive and electroactive properties of meshes coated with reduced graphene allow the potential application of local electric fields or local ionic ...
大小:1795 kb
页数:11 PAGES
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目录:
  • Fabrication of electrospun silk fibroin scaffolds coated with graphene oxide and reduced graphene for applications in biome...
    • 1. Introduction
    • 2. Material and methods
      • 2.1. Silk fibroin processing
      • 2.2. Electrospinning and post-treatment of the mats
      • 2.3. Graphene oxide coating and in situ reduction
      • 2.4. Scanning electron microscopy (SEM)
      • 2.5. Characterisation of mechanical properties of electrospun mats
      • 2.6. Attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR)
      • 2.7. Electrochemical characterisation of the RG-coated meshes
      • 2.8. Cell culture
        • 2.8.1. Routine culture of the L929 cell line
        • 2.8.2. Sterilisation and plating technique of the mats
      • 2.9. Measurement of cell proliferation. MTT assay
      • 2.10. Statistical analysis
    • 3. Results and discussion
      • 3.1. SEM and fibre sizes of the electrospun mats
      • 3.2. Characterisation of mechanical properties of electrospun mats
      • 3.3. Voltammetric characterisation of meshes coated with reduced graphene
      • 3.4. Chronoamperometric characterisation of reduced graphene coated meshes
      • 3.5. ATR-FTIR
      • 3.6. Cell culture experiments. Adhesion and proliferation assay
    • 4. Conclusions
    • Acknowledgements
    • Appendix A. Supplementary data
    • References

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