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标题:Rapid Detection of Staphylococcus aureus in Food Using a Recombinase Polymerase Amplification-Based Assay
时间:2019-04-15 21:52:05
DOI:10.1007/s12161-018-1267-1
作者:Yunyun Geng;Siying Liu;Jinfeng Wang;Huizhu Nan;Libing Liu
出版源: 《Food Analytical Methods》 ,2018 :1-10
摘要:Staphylococcus aureus ( S. aureus) is of great importance and is a leading cause of food poisoning, which is a public health concern in terms of the frequency and seriousness of the disease. In the...
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目录:
  • Rapid Detection of Staphylococcus aureus in Food Using a Recombinase Polymerase Amplification-Based Assay
    • Abstract
    • Introduction
    • Material and Methods
      • Bacterial Strains
      • Generation of Standard DNA
      • Primers and Exo Probes
      • RPA Assay and Real-time RPA Assays
      • Real-time PCR
      • Analysis of Analytical Specificity and Sensitivity
      • Artificial Inoculation of S. aureus into Powdered Infant Formula (PIF), Pork, Steamed Bun, Codfish, and Orange Juice Samples
    • Results
      • Analytical Specificity and Sensitivity of the RPA Assay for the Detection of S. aureus
      • Analytical Specificity and Sensitivity of the Real-time RPA Assay
      • Validation with Artificially Contaminated Samples
    • Discussion
    • Conclusion
    • References

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