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标题:Inhibition of glycine transporter-1 in the dorsal vagal complex improves metabolic homeostasis in diabetes and obesity.
时间:2019-04-15 21:50:45
DOI:10.1038/ncomms13501
PMID:27874011
作者:Jessica T. Y. Yue;Mona A. Abraham;Paige V. Bauer;Mary P. LaPierre;Peili Wang
出版源: 《Nature Communications》 ,2016 ,7 :13501
摘要:Inhibition of glycine transporter-1 in the dorsal vagal complex improves metabolic homeostasis in diabetes and obesityGlycine sensing in the dorsal vagal complex (DVC) regulates hepatic glucose production in rodents.doi:10.1038/ncomms13501Jessica T. Y. YueMona A. AbrahamPaige V. BauerMary P. LaPierre...
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目录:
  • title_link
    • Results
      • Gluco-regulation by DVC GlyT1 inhibition in healthy rodents
    • Figure™1Chemical inhibition of DVC GlyT1 regulates glucose homeostasis in healthy rats.(a) Schematic representation of working hypothesis: glycine™transporter-1 (GlyT1) facilitates the cellular uptake of glycine in the dorsal vagal complex (DVC). Chemical
      • Anti-diabetic effect of DVC GlyT1 inhibition
    • Figure™2Molecular inhibition of DVC GlyT1 regulates glucose homeostasis in healthy rats.(a) Representative western blots and protein levels of plasma membrane GlyT1 (55, 70 and 90thinspkDa isoforms) normalized to insulin receptor (IR) in DVC wedges of rat
      • Metabolic benefits of DVC GlyT1 inhibition in obesity
    • Figure™3DVC and intravenous infusion of ALX regulates glucose homeostasis in 3d-HFD rats.(a) Experimental protocol for b-c. (b) Glucose infusion rates and (c) glucose production during clamps with DVC infusion of saline (n=5), glycine (n=6), ALX (n=5), gl
    • Figure™4Inhibition of DVC GlyT1 regulates glucose homeostasis in diabetic rats.(a) Experimental protocol for b-c. (b) Plasma levels of glucose in overnight-fasted 7d STZsolNicsolHFD diabetic rats (black bars, n=17) compared with non-diabetic, regular chow
      • DVC GlyT1 inhibition regulates energy balance
    • Figure™5Chemical inhibition of DVC GlyT1 regulates glucose homeostasis in obese rats.(a) Experimental protocol for b-d. (b) Body weight gain in rats that were fed with HFD (white circles, n=18) or regular chow (RC, white squares, n=6). Inflections of the
    • Figure™6Molecular inhibition of DVC GlyT1 regulates metabolic homeostasis in obese rats.(a) Experimental protocol for b-e. (b) Glucose infusion rates and (c) glucose production during clamps in 28-d HFD-fed rats with DVC lentivirus (LV) injection of GlyT1
    • Discussion
    • Figure™7Chemical and molecular inhibition of DVC GlyT1 regulates energy balance.(a) Experimental protocol for feeding experiments in rats that received DVC injection of glycine (black squares, n=11) or saline (white squares, n=11). (b) Cumulative food int
    • Methods
      • Animal preparation and surgical procedures
      • Intravenous glucose tolerance test
      • Pancreatic basal insulin euglycaemic clamp in rats
      • Hepatic branch vagotomy in rats
      • Microdialysis
      • DVC virus injection
      • Brain tissue sampling in rats
      • Microdialysate sample glycine analysis
      • Acute (3-day) and chronic (28-day) high-fat feeding in rats
      • Intravenous ALX infusion clamps
      • Induction of experimental type 2 diabetes
      • Fasting-refeeding experiments
      • Pancreatic basal insulin euglycaemic clamp in mice
      • Western blot analyses
      • Biochemical analysis
      • Calculations and statistics
      • Data availability
    • NgM.Global, regional, and national prevalence of overweight and obesity in children and adults during 1980-2013: a systematic analysis for the Global Burden of Disease Study 2013Lancet3847667812014Global report on diabetesWorld Health Organization, Geneva
    • We are grateful to Elena Burdett and Penny Y.T. Wang for technical assistance. This work was supported by a Canadian Institutes of Health Research (CIHR) Foundation Grant to T.K.T.L. (FDN-143204) and the Yale Diabetes Research Centre (P30 DK45735). J.T.Y
    • ACKNOWLEDGEMENTS
    • Author contributions
    • Additional information

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