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标题:Megalin mediates plasma membrane to mitochondria cross-talk and regulates mitochondrial metabolism.
时间:2019-03-15 02:59:31
DOI:10.1007/s00018-018-2847-3
PMID:29916093
作者:Q Li;F Lei;Y Tang;JS Pan;Q Tong
出版源: 《Cellular & Molecular Life Sciences》 ,2018 :1-20
摘要:Megalin mediates plasma membrane to mitochondria cross-talk and regulates mitochondrial metabolism.Mitochondrial intracrines are extracellular signaling proteins, targeted to the mitochondria. The pathway for mitochondrial targeting of mitochondrial ...
大小:7150 kb
页数:21 PAGES
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目录:
  • Megalin mediates plasma membrane to mitochondria cross-talk and regulates mitochondrial metabolism
    • Abstract
    • Introduction
    • Results
      • Megalin is present in the mitochondria
      • Megalin associates with STC1 and SIRT3
      • Mitochondrial targeting of extracellular STC1 is megalinLrp2 dependent
      • Mitochondrial targeting of extracellular rSTC1, a biological phenomenon shared among many cells
      • Megalin mediates trafficking and mitochondrial targeting of the intracrines angiotensin II and TGF-β
      • Retrograde endosome-to-Golgi pathway mediates targeting of mitochondrial intracrines
      • Knockdown of megalin diminishes respiratory and glycolytic capacities
    • Discussion
    • Materials and methods
      • Cells and reagents
      • Generation and purification of rSTC1-FLAG protein
      • sgRNA knockout of megalinLRP2 in C2C12 cells
      • Immunohistochemistry (IHC), immunocytochemistryfluorescence and confocal deconvolution fluorescence
      • Electron microscopy and immunogold labeling
      • Immunoprecipitation and immunoblotting
      • Seahorse XF cell mito stress test
      • Seahorse XF glycolysis stress test
      • Statistical analysis
    • Acknowledgements
    • References

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