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标题:Factors of the bone marrow microniche that support human plasma cell survival and immunoglobulin secretion
时间:2019-01-12 00:15:02
DOI:10.1038/s41467-018-05853-7
作者:Nguyen Doan C.;Garimalla Swetha;Xiao Haopeng;Kyu Shuya;Albizua Igor
出版源: Nature Communications
摘要:Factors of the bone marrow microniche that support human plasma cell survival and immunoglobulin secretiondoi:10.1038/s41467-018-05853-7Nature CommunicationsNguyen Doan C.Garimalla SwethaXiao HaopengKyu ShuyaAlbizua IgorGalipeau JacquesChiang Kuang-YuehWaller Edmund K.Wu RonghuGibson GregRoberson JamesLund Frances E....
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目录:
  • Factors of the bone marrow microniche that support human plasma cell survival and immunoglobulin secretion
    • Results
      • MSC co-cultures support short-term ASC survival
      • Long-term survival and function of ASC in MSC co-cultures
      • Cell–nobreakcell contact is not required for ASC survival
      • MSC secretomes restore the secretory function of sorted ASC
      • APRIL enhances ASC survival induced by the MSC secretome
      • Proteomics of the MSC secretome
      • Role of mTORC1’signaling in LLPC maturation
      • ASC survival is enhanced under hypoxic conditions
    • Discussion
    • Methods
      • Human subjects
      • BM-derived mesenchymal stromal cells and secretome
      • Isolation of PBMC and FACS-based purification of blood ASC
      • In vitro culture systems for human blood and BM ASC
      • Elispot assays
      • BrdU cell proliferation assays
      • Proteomics
      • Peptide purification and LC-MS/MS analysis
      • RNA transcriptomes of blood and BM ASC
      • Bioinformatics
      • Statistical analyses
      • References
    • References
    • Acknowledgements
    • Author contributions
    • Competing interests
    • ACKNOWLEDGEMENTS

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