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标题:Enhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathway.
时间:2019-01-11 21:14:11
DOI:10.1038/s41467-018-07859-7
PMID:30626868
大小:3758 kb
页数:19 PAGES
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目录:
  • Enhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathway
    • Results
      • Synthesis and anti-inflammatory activities of UroA and UAS03
      • UroA/UAS03 induce tight junction proteins
      • AhR mediates the activities of UroA/UAS03
      • UroA/UAS03 enhance gut barrier function through Nrf2
      • Treatment with UroA/UAS03 mitigates colitis
      • UAS03/UroA mediated protection against colitis requires AhR-Nrf2 pathways
    • Discussion
    • Methods
      • Materials
      • Mice
      • Synthetic procedure for synthesis of UAS03
      • Cell cultures
      • Measurements of IL-6 and TNF-α levels in BMDM
      • LPS-induced peritonitis
      • Real-time PCR
      • In vitro permeability study
      • RNA sequencing
      • Immunoblots (western blots)
      • Confocal imaging
      • AhR-reporter assay
      • Nrf2-reporter assay
      • Measurements of Cyp1A1 enzyme activty (ex’vivo)
      • Ethoxyresorufin-O-deethylase (EROD) assay
      • P450-Glo Cyp1A1 luminiscence assay
      • Measurement of Cyp1A1 enzyme activities in’vitro
      • Small interfering RNA (siRNA) mediated knockdown experiment
      • Cyp1A1 deletion by CRISPR/Cas9 method
      • NF-κB EMSA assay
      • Colon explant culture
      • Tissue processing for RNA and protein analysis
      • 28-day repeated dose toxicity study
      • 2,4,6-Trinitrobenzenesulfonic acid (TNBS)-induced colitis
      • DSS-induced colitis
      • Assessment of colitis severity and tissue collection
      • In vivo intestinal permeability assay
      • Myeloperoxidase (MPO) activity
      • Histopathology
    • References
    • References
    • Acknowledgements
    • Author contributions
    • Competing interests
    • ACKNOWLEDGEMENTS

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