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标题:Icariin improves Fanconi anemia hematopoietic stem cell function through SIRT6-mediated NF-kappa B inhibition.
时间:2019-01-11 20:46:53
DOI:10.1080/15384101.2018.1426413
PMID:29355456
作者:Y Li;X Li;A Cole;S McLaughlin;W Du
出版源: 《Cell Cycle》 ,2018 ,17 (3) :1
摘要:Hematopoietic stem cells (HSCs) preserve their ability to self-renew and differentiate to different lineages in the bone marrow (BM) niche, which is composed in large part by BM stromal cells. Studies have shown that altered signaling in...
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目录:
  • Abstract
  • Introduction
  • Results
    • ICA restores quiescence of FA HSCs
    • ICA improves FA HSC function
    • ICA upregulates SIRT6 enzyme activity
    • Upregulated SIRT6 by ICA inhibits NF-κB in FA HSPCs
  • Discussion
  • Materials and methods
    • Mice and treatments
    • Flow cytometry analysis
    • Isolation of bone marrow cells and BM transplantation
    • Colony-forming unit assay
    • SIRT6 level detection
    • Acetylation of H3K9
    • Deacetylation of Histone H3 Lysine 9 on the promoter of NF-κB (RelA)
    • RNA Isolation, Reverse Transcriptase (RT)-PCR
    • Plasmids and antibodies
    • Statistical analysis
  • Acknowledgments
  • Disclosure of Potential Conflicts of Interest
  • Funding
  • References

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