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标题:FLNa negatively regulated proliferation and metastasis in lung adenocarcinoma A549 cells via suppression of EGFR.
时间:2018-12-07 09:19:41
DOI:10.1093/abbs/gmx135
PMID:29272322
作者:Zhang Y;Zhu T;Liu J;Liu J;Gao D
出版源: 《Acta Biochim Biophys Sin》 ,2018 ,50 (2)
摘要:FLNa negatively regulated proliferation and metastasis in lung adenocarcinoma A549 cells via suppression of EGFRAbstract Filamin A (FLNa) is a ubiquitously expressed cytoplasmic protein, which composes of an N-terminal actin binding domain (...
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目录:
  • FLNa negatively regulated proliferation and metastasis in lung adenocarcinoma A549 cells via suppression of EGFR
    • Introduction
    • Materials and Methods
      • Cell culture
      • RNA isolation and quantitative real-time PCR
      • Protein extraction and western blot analysis
      • Lentivirus production and stable cell line selection
      • MTT cell proliferation assay
      • Immunoprecipitation assay
      • Scratch wound-healing assay
      • Migration assay
      • Invasion assay
      • Tumorigenesis study
      • Immunohistochemical staining
      • Statistical analysis
    • Results
      • Silencing FLNa enhances proliferation capacity of A549 cells
      • FLNa silencing promotes migration capacity of A549 cells
      • FLNa silencing increases invasiveness of A549 cells
      • Role of FLNa in ligand-induced EGFR phosphorylation in human A549 cells
      • Silencing FLNa increases tumorigenic capacity of A549 cells
    • Discussion
    • Funding
    • References

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