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标题:Simultaneous detection of benzimidazole-resistant strains of Fusarium head blight using the loop-mediated isothermal amplification-fluorescent loop primer method
时间:2018-12-07 08:33:59
DOI:10.1007/s10327-018-0788-1
作者:Ryoji Komura;Taku Kawakami;Kaori Nakajima;Hirofumi Suzuki;Chiharu Nakashima
出版源: 《Journal of General Plant Pathology》 ,2018 ,84 (4) :247-253
摘要:Simultaneous detection of benzimidazole-resistant strains of Fusarium head blight using the loop-mediated isothermal amplification-fluorescent loop primer methodThe loop-mediated isothermal amplification (LAMP)-fluorescent loop primer (FLP) method detects genetic polymorphisms by using a LAMP amplicon and measuring the peak temperatures of fluorescence resonance energy transfer between an...
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目录:
  • Simultaneous detection of benzimidazole-resistant strains of Fusarium head blight using the loop-mediated isothermal amplification-fluorescent loop primer method
    • Abstract
    • Introduction
    • Materials and methods
      • Isolates and symptomatic Fusarium head blight samples used in this study
      • PCR reaction and sequencing
      • Design of LAMP primers and probe sets
      • LAMP-FLP reaction
      • Detection of MBC-resistant strains in field samples using the LAMP-FLP analyses
      • Field sample monitoring trial of MBC-resistant strains using the LAMP-FLP method
    • Results
    • Discussion
    • Acknowledgements
    • References

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