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标题:ATP citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the Fusarium tricinctum species complex in pure cultures and in cereal samples.
时间:2018-12-07 08:25:09
DOI:10.1016/j.ijfoodmicro.2012.06.021
PMID:22867849
作者:Niessen L;Gräfenhan T;Vogel RF
出版源: 《International Journal of Food Microbiology》 ,2012 ,158 (3) :171-185
摘要:(2012) ATP citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the Fusarium tricinctum species complex in pure ...
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目录:
  • ATP citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the Fusarium tricinctum species complex in pure cultures and in cereal samples
    • 1. Introduction
    • 2. Materials and methods
      • 2.1. Organisms and media
      • 2.2. DNA preparation
      • 2.3. DNA amplification
      • 2.4. Phylogenetic analyses
      • 2.5. Direct analysis of fungal agar cultures, single barley grains, and bulk grain samples
    • 3. Results
      • 3.1. Primer design
      • 3.2. Optimization of LAMP reaction conditions with primer set tric acl1 ID13 and testing of specificity and sensitivity
      • 3.3. Application of the F. tricinctum specific LAMP assay
      • 3.4. Application of simple lab equipment to incubate the LAMP reaction
    • 4. Discussion
    • Acknowledgments
    • References

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