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标题:Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Fusarium oxysporum f. sp. cubense race 4
时间:2018-12-07 08:23:02
DOI:10.1007/s10658-012-0136-9
作者:Li;Benjin;Du;Jialiang;Chengzhong
出版源: 《European Journal of Plant Pathology》 ,2013 ,135 (4) :903-911
摘要:Li B, Du J, Lan C, Liu P, Weng Q, Chen Q (2013) Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Fusarium oxysporum f. sp. cubense race 4. Eur J Plant Pathol 135:903–911 View ArticleLi B, Du J, Lan C, Liu P, Weng Q, Chen Q (2013a) Development of a loop-mediated isothermal amplification assay for...
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目录:
  • Development...
    • Abstract
    • Introduction
    • Materials and methods
      • Fungal isolates and DNA extraction
      • Detection of Foc race 4 by PCR
      • LAMP primer design
      • LAMP reaction and detection
      • Specificity and sensitivity of LAMP
      • LAMP testing of diseased banana
    • Results
      • Detection of Foc race 4 using PCR
      • Optimization of LAMP assay
      • LAMP assay specificity
      • Detection limit of LAMP assay
      • Evaluation of the LAMP assay using diseased plant material
    • Discussion
    • References

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