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标题:Rapid diagnosis of Fusarium root rot in soybean caused by Fusarium equiseti or Fusarium graminearum using loop-mediated isothermal amplification (LAMP) assays
时间:2018-12-07 08:14:51
DOI:10.1007/s13313-015-0361-8
作者:C. Lu;H. Zhang;Y. Wang;X. Zheng
出版源: 《Australasian Plant Pathology》 ,2015 ,44 (4) :437-443
摘要:ABSTRACT Rapid diagnostic assays for Fusarium root rot in soybean, caused by Fusarium equiseti or Fusarium graminearum were developed using the target gen...
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目录:
  • Rapid...
    • Abstract
    • Introduction
    • Materials and methods
      • Strain sources
      • Culture conditions and DNA extraction
      • LAMP primer design and selection
      • LAMP reaction
      • Detection of the LAMP product
      • DNA extraction from soil containing F.’equiseti or F.’graminearum conidia
      • DNA extraction from diseased tissues
    • Results
      • Specificity of the CYP51C-FE-LAMP assay and the CYP51C-FG-LAMP assay
      • Sensitivity of the CYP51C-FE-LAMP assay and the CYP51C-FG-LAMP assay
      • Evaluation of the CYP51C-FE-LAMP assay and the CYP51C-FG-LAMP assay detecting F.’equiseti and F.’graminearum in diseased soybean tissues
    • Discussion
    • References

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