| 标题： | Rapid diagnosis of Fusarium root rot in soybean caused by Fusarium equiseti or Fusarium graminearum using loop-mediated isothermal amplification (LAMP) assays |
| 时间： | 2018-12-07 08:14:51 |
| DOI： | 10.1007/s13313-015-0361-8 |
| 作者： | C. Lu;H. Zhang;Y. Wang;X. Zheng |
| 出版源： | 《Australasian Plant Pathology》 ,2015 ,44 (4) :437-443 |
| 摘要： | ABSTRACT Rapid diagnostic assays for Fusarium root rot in soybean, caused by Fusarium equiseti or Fusarium graminearum were developed using the target gen... |
| 大小： | 875 kb |
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目录：
- Rapid...
- Abstract
- Introduction
- Materials and methods
- Strain sources
- Culture conditions and DNA extraction
- LAMP primer design and selection
- LAMP reaction
- Detection of the LAMP product
- DNA extraction from soil containing F.equiseti or F.graminearum conidia
- DNA extraction from diseased tissues
- Results
- Specificity of the CYP51C-FE-LAMP assay and the CYP51C-FG-LAMP assay
- Sensitivity of the CYP51C-FE-LAMP assay and the CYP51C-FG-LAMP assay
- Evaluation of the CYP51C-FE-LAMP assay and the CYP51C-FG-LAMP assay detecting F.equiseti and F.graminearum in diseased soybean tissues
- Discussion
- References
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