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标题:Tumor Evolution and Drug Response in Patient-Derived Organoid Models of Bladder Cancer.
时间:2018-12-06 22:49:14
DOI:10.1016/j.cell.2018.03.017
PMID:29625057
作者:SH Lee;W Hu;JT Matulay;MV Silva;TB Owczarek
出版源: 《Cell》 ,2018 ,173 (2) :515-528.e17
摘要:Bladder cancer is the fifth most prevalent cancer in the U.S., yet is understudied, and few laboratory models exist that reflect the biology of the human disease. Here, we describe a biobank of patient-derived organoid lines that recapit...
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页数:33 PAGES
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目录:
  • Tumor Evolution and Drug Response in Patient-Derived Organoid Models of Bladder Cancer
    • Introduction
    • Results
      • Establishment of Patient-Derived Bladder Cancer Organoid Lines
      • Organoid Lines Recapitulate the Mutational Spectrum of Human Bladder Cancer
      • Tumor Evolution in Organoid Culture
      • Phenotypic Stability and Plasticity in Organoid Culture
      • Classification of Organoid Lines into Basal and Luminal Subtypes
      • Drug Response of Organoid Lines
    • Discussion
    • Supplemental Information
    • Acknowledgments
    • References
    • STAR★Methods
      • Key Resources Table
      • Contact for Reagent and Resource Sharing
      • Experimental Model and Subject Details
        • Human specimens
        • Patient clinical characteristics
        • Organoid xenograft studies
      • Method Details
        • Sample Collection
        • Tissue dissociation and organoid culture
        • Histology and immunostaining
        • Targeted sequencing
        • Whole-exome sequencing
        • Sequence alignment and mutation identification
        • Mutational signatures and phylogenetic analysis
        • Detection of FGFR3-TACC3 fusion transcript
        • Gene expression profiling
        • Organoid drug response assay
        • Western blotting
        • Organoid xenograft studies
      • Quantification and Statistical Analysis
      • Data and Software Availability

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