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标题:Purification and characterization of an esterase isozyme involved in hydrolysis of organophosphorus compounds from an insecticide resistant pest, Helicoverpa armigera (Lepidoptera: Noctüidae).
时间:2018-12-06 22:14:35
DOI:10.1016/j.bbagen.2005.12.009
PMID:16466860
作者:Srinivas, R;Jayalakshmi, SK;Sreeramulu, K;Sherman, NERao, J
出版源: BBA - General Subjects ,2006 ,1760 (3) :310-317
摘要:Rao J.Purification and characterization of an esteraseisozyme involved in hydrolysis of organophosphorus compounds from an insecticide resistant pest,Helicoverpa...
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目录:
  • Purification and characterization of an esterase isozyme involved in hydrolysis of organophosph.....
    • Introduction
    • Materials and methods
      • Insects
        • Chemicals
      • Extraction of esterase
      • Enzyme assay
      • Purification of the esterase
      • Electrophoresis
      • Effect of pH and temperature
      • Effect of metal ions and inhibitors
      • Micro-sequencing of the enzyme
    • Results
      • Enzyme purification
      • Catalytic properties
      • Inhibition
      • Amino acid sequence analysis
    • Discussion
    • Acknowledgments
    • References

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