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标题:Deciphering genome-wide WRKY gene family of Triticum aestivum L. and their functional role in response to Abiotic stress.
时间:2018-11-08 22:17:01
DOI:10.1007/s13258-018-0742-9
PMID:30238225
作者:Saurabh Gupta;Vinod Kumar Mishra;Sunita Kumari;Raavi;Ramesh Chand
摘要:Deciphering genome-wide WRKY gene family of Triticum aestivum L. and their functional role in response to Abiotic stressWRKY transcription factors (TFs) act in regulating plant growth and development as well as in response to different stress. Some earlier studies done by individual researchers reported different......
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目录:
  • Deciphering genome-wide WRKY gene family of Triticum aestivum L. and their functional role in response to Abiotic stress
    • Abstract
    • Introduction
    • Material and methodology
      • Identification of WRKY genes and its chromosomal location analysis
      • Phylogenetic analysis and estimation of KaKs
      • Modeling and TaWRKYs ‘W-box’ of DNA interaction analysis
      • Microarray-based analysis of heat andor drought-responsive genes
      • Gene ontology annotation and enrichment analysis
      • Extraction of genomic DNA from young leaves of wheat and target genes primer synthesis
      • Initial amplification of heat orand induced TaWRKY genes
      • Qualitative gene amplification through multiplex PCR
    • Results
      • Genomic locations and duplication events among the UDS-TaWRKY genes
      • Phylogenetic relationship and structure variations in TaWRKY and TuWRKY TFs
      • Molecular interaction analysis of TaWRKYand ‘W-Box’DNA complex
      • Expression analysis of heat andor drought genes during early grain development of wheat
      • GO annotation analysis
      • In-vitro qualitative detection of heat andor drought TaWRKY genes
    • Discussion
      • TaWRKY TFs and their organization
      • Phylogenetic classification of TaWRKY TFs
      • Functional role of TaWRKYs in different stress conditions
    • Conclusion
    • References

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