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标题:Molecular cloning and functional characterization of spexin in orange-spotted grouper (Epinephelus coioides).
时间:2018-11-08 21:47:13
DOI:10.1016/j.cbpb.2016.02.009
PMID:26944307
作者:Li S;Liu Q;Xiao L;Chen H;Zhang Y
出版源: 《Comparative Biochemistry & Physiology Part B B... ,2016 ,196-197 :85-91
摘要:Molecular cloning and functional characterization of spexin in orange-spotted grouper (Epinephelus coioides).SpexinReproductionFeeding controlOrange-spotted grouperSpexin is a newly discovered neuropeptide in vertebrates. Comprehensive comparative studies are required to unveil its biological functions. In order to ascertain the neuroendocrine function ofspexinin orange-spotted grouper, ...
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目录:
  • Molecular cloning and functional characterization of spexin in orange-„spotted grouper (Epinephelus coioides)
    • 1. Introduction
    • 2. Materials and methods
      • 2.1. Animals
      • 2.2. Cloning and sequencing of cDNA and genomic DNA
      • 2.3. Tissue distribution of spexin expression
      • 2.4. Hypothalamic expression of spexin at different gonadal stages
      • 2.5. Hypothalamic expression of spexin after food deprivation
      • 2.6. Effects of spexin-14 on the expression of appetite-related genes, gonadotropin-releasing hormone 1 and pituitary hormones
      • 2.7. Quantitative real-time PCR
      • 2.8. Statistical analysis
    • 3. Results
      • 3.1. Analysis of orange-spotted grouper spexin cDNA and genomic DNA sequence
      • 3.2. Tissue expression pattern of spexin in orange-spotted grouper
      • 3.3. Expression pattern of spexin during the ovarian development of orange-spotted grouper
      • 3.4. Effect of food deprivation on spexin expression in orange-spotted grouper
      • 3.5. In vivo effects of spexin-14 on the mRNA expression of appetite-related genes, gnrh1 and pituitary hormones
      • 3.6. In vitro effects of spexin-14 on the mRNA expression of pituitary hormones
    • 4. Discussion
    • Acknowledgments
    • References

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