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标题:Expression of the transient receptor potential vanilloid 1 (TRPV1) in LNCaP and PC-3 prostate cancer cells and in human prostate tissue.
时间:2018-10-11 17:58:48
DOI:10.1016/j.ejphar.2005.04.010
PMID:15913603
大小:287 kb
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目录:
  • Expression of the transient receptor potential vanilloid 1 (TRPV1) in LNCaP and PC-3 prostate cancer cells and in human prostate tissue
    • Introduction
    • Materials and methods
      • Cell cultures
      • Human prostate tissue
      • Isolation of total RNA, reverse transcriptase polymerase chain reaction (RT-PCR) and DNA electrophoresis
      • Western blot analysis
      • [125I]RTX binding assays
      • Measurement of intracellular calcium concentration [Ca2+]i
      • Statistical analysis
    • Results
      • Expression of the vanilloid TRPV1 receptor in prostate cells and prostate tissue
      • Characterization of [125I]-resiniferatoxin receptor binding
      • Vanilloids induce an increase in [Ca2+]i in LNCaP and PC-3 cells
    • Discussion
    • Acknowledgements
    • References

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